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From 2010.igem.org

Lux inverter

2010-10-8

transformation

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BBa_R0061 BBa_E0240

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2010-10-9

Mini-prep BBa_R0061 BBa_E0240

digestion

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BBa_R0061 20 µL 10×Buffer 3 µL SpeI　　　　　 1 µL PstI　　　　　 1 µL dH₂O5 µL

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BBa_E0240 20 µL 10×Buffer 3 µL XbaI 1 µL PstI　　　　　 1 µL dH₂O 5 µL

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gel extraction

2010-10-11

Ligation

R0061 (70 ng) 1 µL E0240 (100 ng) 2 µL ATP 1 µL 10×Buffer 1 µL T4 DNA Ligase 1 µL dH₂O 4 µL

transformation

2010-10-12

Ligation

R0061(100 ng) 1 µL E0240 (100 ng) 3 µL ATP (10 mM) 1 µL T4 DNA Ligase 1 µL dH₂O 4 µL

2010-10-13