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From 2010.igem.org

Nina

Preparation of competent DH5α Escherichia coli cells

Continued from 21/6.

Prepared competent DH5α cells according to the protocol of Morten Nørholm at the Department of Biochemistry & Biophysics Stockholm University.

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Absorbance:

• 1 ml sample from one bottle:

Time				OD
30min				0,029
1h				0,053
1h				0,188
30min				0,316

• 1 ml sample from two bottles:

Time				OD
30min				0,519/0,503
7min				0,578/0,566
5min				0,598/0,600