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From 2010.igem.org

The Story So Far

30th June

We found the T.aquaticus DNA polymerase sequence from the NCBI website. From this, we designed primers that contained restriction site "sticky ends" on the 5' and 3'. The restriction sites that are going to be used for introducing Taq into the vector are XbaI (5') and SpeI (3').

Forward primer:

5' CTA GAA TGA GGG GGA TGC TGC CCC TCT TTG AG 3'

Length: 32
GC Content: 56.3 %
Tm: 65.6 ºC

Reverse Primer:

5' GAT CAT CAC TCC TTG GCG GAG AGC CAG TCC 3'

Length: 30
GC Content: 60.0 %
Tm: 66.2 ºC

29th June

The team is starting to design the experiment. We need to:

• Design primers to introduce Taq into psB1C3
• Create a good protein expression vector.

22th June

Work has begun on creating primers to extract the protein we are working on from its wild-type plasmid into a plasmid which conforms to biobrick cloning standards.

19th June

Jeremy Nagels, a student at Monash university, Ebony and Symon have begun discussing various project ideas. These include:

Oscillators

The use of the Hydrogenase 3 complex to generate Hydrogen within E Coli as a form of biofuel.

The possibility of biological prevention of tooth decay with biobricks.

The application of biobricks to a mixed cell community.

The development of cell free transcription/ translation within a cellular system, for example the phloem of a plant, (although the development of this level of symbiosis may be the stuff of future projects)

The attempt is being made to develop a project that in some ways reflects these interests and possibly taps a common thread underlying them.