Team:Kyoto/Project/Goal C

Goal C: Characterization of the anti-killer gene

Introduction

We checked the function of the anti-killer gene. E.coli transformed with the constructs below was grown in medium without IPTG and IPTG was added to the culture at proper time. The A550 of the culture was measured and the result was compared with that of the experiment of the killer gene in order to find whether the anti-killer gene works correctly.

Method

Bacterial strains

We used three types of E. coli, E. coli KRX transformed with <partinfo>BBa_K358021</partinfo>, KRX transformed with <partinfo>BBa_K358024</partinfo>, KRX transformed with <partinfo>BBa_K358022</partinfo>.

Measurement

We picked up three colonies from each plate, and cultivated them in M9 medium overnight. The overnight cultures were diluted to 0.1-0.12 (OD600) by M9 medium.After that, we added several amounts of IPTG to the cultures and measured OD600 of each culture at several points.

Result

Discussion