An Integrated Platform Based on Bacterial Microcompartment for de novo Proteinaceous Artificial Organelles
Part Ⅱ: Fusion Proteins for Transportation into BMC
It is reported that a short N-terminal peptide is necessary and sufficient for packing enzymes into the lumen of the BMC. Fusion of the 14, 18 or 64 N-terminal amino acids from PduP to GFP or RFP resulted in their encapsulation within BMCs. Each fusion protein (PduP1-14-GFP, PduP1-18-GFP, PduP1-64-GFP) was produced by the new standard. When 14/18/64 amino acids from the N terminus of PduP were fused to GFP, the fusion protein was readily detected by Western blotting. These results, in conjunction with the above studies, indicate that a short region of the N terminus of PduP is necessary and sufficient for packing proteins to the lumen of the BMC.
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