Team:Groningen/Biobricks

From 2010.igem.org

Biobricks

Here is our registry of our biobricks: <groupparts>iGEM010 Groningen</groupparts>

Summary:

Below is given a short description of all the biobricks in our registry.

Chaplins

BBa_K305001 Hydrophobic protein chaplin C (chpC)

Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.

BBa_K305002 chaplin C (chpDC) with modified sortase binding motive

Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.

Modified C-terminal Sortase(SrtA from S. aureus.) recognition site.

BBa_K305003 Hydrophobic protein chaplin E (chpE)

Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.

BBa_K305004 Hydrophobic protein chaplin H (chpH)

Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.

BBa_K305005Sortase srtA

Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall

Promotors

BBa_K305006 yqxMpr

yqxM promotor effective in biofilm formation, regulates tasA production

BBa_K305007 srfApr 1

Promotor for the surfactin regulatory gene.

BBa_K305008 srfApr 2

Promotor for the surfactin regulatory gene.

BBa_K305009 srfApr1>GFP

Promotor for the surfactin regulatory gene with GFP.

BBa_K305010 srfApr2>GFP

Promotor for the surfactin regulatory gene with GFP.

Plasmid Backbone

BBa_K305011 Subtilin inducible expression plasmid for Bacillus subtilis

Adapted from the system for subtilin-regulated gene expression (SURE) in Bacillus subtilis [1], this plasmid was constructed to express proteins present in RCF 10 BioBricks upon subtilin induction.