Team:Yale/Our Project/Protocols/bacteria mixture for H2S Assay

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Bacteria Mixture for H2S Assay

In order to facilitate the manipulation of E.coli, a solution was made using:

  • 10 microliters of IPTG
  • 200 microliters of S.O.C. (a medium that promotes growth)
  • E.coli cultures


This mixture was then vortexed to create a thick liquid of bacteria.