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From 2010.igem.org

Date: 06/28/2010

• A. Ann, Bryce, and I had a short meeting with Mike (Graduate Student Advisor) to discuss about HPLC column.
• B. O/N Cell culture (E. Coli / DH5A )

O/N Cell culture

• 1. Put 2ml LB into 15ml test tube (for incubation.
• 2. Get a stock of DH5A stored in -80C freezer.
• 3. Use a pipette tip and scratch the surface of the stock, then put the pipette tip into the 15ml test tube.
• 4. Place the test tube into 30C incubator for O/N.

Note: In order to carry out the procedure in sterile environment, clean the lab table with EtOH and turn on the flame. Make sure to work near the flame and sterilize the test tube opening and its cap with the flame quickly. When transfer the stock, use a new pair of gloves. In addition, -80 freezer must not be opened for more than 20 seconds.