Team:Imperial College London/Modelling/Output/Objectives

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Output Amplification Model

Objectives

Our novel concept of amplifying our output by incorporating enzymes raised some important questions that needed to be answered:
  1. How beneficial is it to incorporate the enzyme amplification step? We need to compare the speed of response between transcription and translation with 1-, 2- or 3-step amplification.
  2. How many amplification steps are beneficial? Will the addition of further amplification steps introduce considerable time delays?
  3. For how many amplification steps addition of another one does not reduce the speed of response very much?
  4. Which enzymes should be used as 3rd amplifying step? TEV (common enzyme for both 2 and 3 amplification step) or HIV1 (all three enzymes at three amplification levels are different)?

In order to answer these questions, we would have to model this system. The results of the computer models would enable us to decide which design would be the most efficient one. This design would then be put forward to be built in labs.

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