Characterisation data is stored in the Parts Registry. Click on any BioBrick to view this data.

Featured parts

BBa_K325219

Red luciferase and LRE operon from L. cruciata under pBAD This part generates the luciferase and luciferin regenerating enzyme proteins from the Japanese firefly, Luciola cruciata, when induced by L-arabinose. The luciferase produced has a point mutation Ser286Asn which gives it a redshifted emission spectrum as compared to wild-type. The sequence of nucleotides is codon optimised for expression in E. coli.

BBa_K325909

V. fischeri luxCDABE under pBAD This part generates luxCDABE proteins from the bioluminescent bacterium, Vibrio fischeri, when induced by L-arabinose. The BioBrick produces light from basic metabolites found in E. coli, luxCDE produce the substrate used by luxAB for bioluminescence. The part creates a blue light.

Parts based on bacterial systems

BBa_K325902	V. fischeri luxCD This is a translational unit designed to produce V. fischeri luxC and luxD genes when placed under a suitable promoter. The DNA sequence is codon optimised for expression in E. coli. Both proteins are part of the fatty aldehyde synthesis pathway. luxC is a reductase and luxD is a acytransferase. LuxE must also be supplied to complete the pathway for fatty aldehyde synthesis. Such fatty aldehydes are the substrate for the luxAB bacterial luciferase.
BBa_K325903	V. fischeri luxEG This is a translational unit designed to produce V. fischeri luxE and luxG genes when placed under a suitable promoter. The DNA sequence is codon optimised for expression in E. coli. luxE functions as the synthetase creating fatty aldehydes for bioluminescence. LuxG is a flavin reductase.
BBa_K325905	luxCDEG pLambda AB This part constitutively expresses <a href=http://partsregistry.org/wiki/index.php?title=Part:BBa_K216008"> the Xenorhabdus luminescens luxAB luciferase</a>. It also features a translational unit for the substrate generating luxCDEG. This means that only when supplied with PoPs will it produce light output.
BBa_K325906	pBAD luxCDEG pLambda AB This is the same as the part above but was built for testing induction by adding the pBAD promoter, making light inducible by addition of L-arabinose.

<groupparts>iGEM010 Cambridge</groupparts>