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Team:Groningen/23 August 2010
From 2010.igem.org
Hydrophobofilm
pushing coatings into a greener future
Expression experiment - David & Peter
The second expression experiment.
Analasys was done by THT and SDS page.
Biofilm formation experiment
Bacillus Subtilis cultures were grown overnight in a Petridish containing 20mL of LB medium and a piece of ceramics to spurr the biofilm formation. The following cultures wehre grown: Bacillus Subtilis, Bacillus Subtilis ΔTasA, Bacillus Subtilis wit chaplin E1, Bacillus Subtilis with chaplin H1, Bacillus Subtilis ΔTasA with chaplin E1, Bacillus Subtilis ΔTasA with chaplin H1
All cultures where inoculated with 100uL of overnight culture. All cultures where run in duplicate, one induced with 1%subtilin, and one without subtilin as a negative control.
We assumed that the ΔTasA would not form a biofilm and the normal phenotype could be restored by the expression of chaplins. Furthermore we assumed that the normal Bacillus Subtilis biofilm would have atered properties.
No such effects could be observed all ΔTasA cultures showed the same non biofilm phenotype
The same goes for all Bacillus Subtilis cultures with TasA, the were all not affected by induction of Chaplins, and showed a normal biofiml forming phenotype.
Our assumption is that either: the expression of chaplins has no visible effect on biofilm formation or, that the subtilin was degraded over night and no proper expression of chaplins took place.
