Team:Groningen/12 July 2010

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iGEM Groningen 2010

Hydrophobofilm
pushing coatings into a greener future

Week 28, Arend Jan


A mix of the ChpE and ChpH genes were ligated into the pNZ8901-bbs backbone and a restriction check was performed on 8 clones. ChpE contains a DpnI site whereas ChpH does not. A restriction with DpnI and XhoI should yield a 487bp and 176bp fragment for ChpE and just a 648bp fragment for ChpH (+ a ~3500bp backbone fragment for both) 

- 10ul plasmid 
- 1.5ul buffer R
- 0.5ul DpnI
- 0.5ul XhoI
- 2.5ul MQ


[[Image:13-07-10gn.jpg|200px|thumb|left|]]
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These ligations are performed with the backbone that still contains unwanted IS element with the PstI site. At the same time we are attempting to delete this element using a related backbone.





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