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Team:Northwestern/Project/Chassis
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Our team primarily used the high transformation efficiency top10 cells from invitrogen for most protocols. In order to ensure lawn formation, the TqsA (also known as the YdgG) knockout were taken from a copy of the Keio Collection with permission from Professor Margaret Saks of Northwestern University. The ΔTqsA strain increases the thickness of biofilm by interfering with the quorum sensing mechanism (more specifically, the autoinducer-2 transport system). To prevent bacteria from digesting synthesized Chitin, as it normally would, the ΔChiA strain was also taken from the Keio Collection. Because this strain lacks Chitinase, a digestive enzyme that breaks the glycosidic bonds in Chitin.
Invitrogen Top10 Cells:Purchase and Description: http://products.invitrogen.com/ivgn/product/C404010 TqsA aka YdgG Knockouts:http://jb.asm.org/cgi/content/short/188/2/587 http://ecoli.aist-nara.ac.jp/GB5/info.jsp?id=JW1593 http://cgsc2.biology.yale.edu/Mutation.php?ID=106356 ChiA Knockouts:http://cgsc2.biology.yale.edu/Mutation.php?ID=100308 http://www.ncbi.nlm.nih.gov/gene/947837 http://ecoli.aist-nara.ac.jp/GB5/info.jsp?id=JW3300 MAGE Protocolhttp://www.wired.com/wiredscience/2009/07/cellfactories/ http://www.nature.com/nature/journal/v460/n7257/full/nature08187.html |
