Team:USTC/Project/safe

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An Integrated Platform Based on Bacterial Microcompartment for de novo Proteinaceous Artificial Organelles

overview

experimental safety

From Feb.4th to Feb.13th, a collective training is held targeting to the practice of basic experiment. Setting GFP protein expression system as an example, we learn from the program how to construct standard components and then an expression system. It`s the time we get to know each other and become a team as a whole.

- The contents of the training are listed as followed:
  - 1. outlines
  - 2. E.coli culture (Medium preparation, Inoculation, Aseptic operation)
  - 3. plasmid extraction
  - 4. PCR
  - 5. DNA Electrophoresis
  - 6. Double Digest
  - 7. reclaim and appraise of the product of enzyme cutting
  - 8. Ligation
  - 9. preparation of competent cell
  - 10. genomic screening of masculine clone
  - 11. DNA sequencing

asking the experts

answering key questions

For iGEM 2010 teams are asked to detail how they approached any issues of biological safety associated with their projects. Specifically, teams should consider the following questions:

Would any of your project ideas raise safety issues in terms of:
- researcher safety,
- public safety, or
- environmental safety?
Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes,
- did you document these issues in the Registry?
- how did you manage to handle the safety issue?
- How could other teams learn from your experience?
Is there a local biosafety group, committee, or review board at your institution?
- If yes, what does your local biosafety group think about your project?
- If no, which specific biosafety rules or guidelines do you have to consider in your country?
Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?