Team:Wisconsin-Madison/experiments

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Enzyme Treatment

Encapsulation

Colonic Acid Quantification

*Parts used in this experiment*
Part Number	Function	Expression Type	Zip File
<partinfo>BBa_k318500</partinfo>	Produces Trascription Factor RcsB	Inducible - IPTG	500
<partinfo>BBa_k318501</partinfo>	Produces Trascription Factor RcsA	Inducible - IPTG	501
<partinfo>BBa_k318502</partinfo>	Produces Trascription Factor RcsA & RcsB	Inducible - IPTG	502	<partinfo>BBa_k200021</partinfo>	Empty vector - control	Inducible - IPTG

See entire procedure : Media:UW_colonicacidassay.pdf Download See original reference: http://pubs.acs.org/doi/abs/10.1021/ja01129a015 Link

Cell Survivability Testing

*Parts used in this experiment*
Part Number	Function	Expression Type	Zip File
<partinfo>BBa_k318500</partinfo>	Produces Trascription Factor RcsB	Inducible - IPTG	500
<partinfo>BBa_k318501</partinfo>	Produces Trascription Factor RcsA	Inducible - IPTG	501
<partinfo>BBa_k318502</partinfo>	Produces Trascription Factor RcsA & RcsB	Inducible - IPTG	502

Best Combination

Inducible-Repressible Expression

Characterize pH Promoters

Parts Used: <partinfo>BBa_k318513</partinfo>

Amount of Regulators

IR-System - Arabinose

Parts Used: <partinfo>BBa_k318509</partinfo>, <partinfo>BBa_k318510</partinfo>, <partinfo>BBa_K318511</partinfo>, <partinfo>BBa_K318506</partinfo>

IR-System - pH

Parts Used: <partinfo>BBa_TUNED Part</partinfo>, <partinfo>BBa_K318506</partinfo>

IR-Lysis - pH

Parts Used: <partinfo>BBa_TUNED Part</partinfo>, <partinfo>BBa_K318507</partinfo>

Bile Induction

<partinfo>BBa_K318516</partinfo>, <partinfo>BBa_Lysis Part</partinfo> <partinfo>BBa_K318516</partinfo>, <partinfo>BBa_LYSIS Part</partinfo>

Encryption

Laboratory Notebooks

Media:Wisconsin-Madison2010_Notebook1.pdf

Media:Wisconsin-Madison2010_Notebook2.pdf

Media:Wisconsin-Madison2010_Notebook3.pdf