Team:DTU-Denmark/Lab protocols

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Ligations

Preparation:

  1. Calculate your ligase concentration by estimation of DNA concentration from your restriction gel.
  2. Which vector to use?

Material:

  1. prepare ligation mix with a total volume of 20 μl
    • 2 μl 10x Buffer
    • Parts to be ligated: vector+insert 1:5, DNA conc. less than 50 ng
    • fill up with water
    • 1 μl ligase
    • control (no insert)
  2. Which vector to use?

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