Construction usu.html

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Utah State University

CyanoBricks

Construction

Figure 2. Construction of M11400 from pIGA. (1) Mutagenesis of EcoRI site. (2) Replacement of GFP cassette with BioBrick prefix and suffix. (3) Mutagenesis of EcoRI and PstI sites. (4) Insertion of NheI digested region from BioBrick Basic Vector.