Restriction Enzyme Digests
From 2010.igem.org
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http://openwetware.org/wiki/Knight:Restriction_Digest
http://openwetware.org/wiki/Endy:Restriction_Digest
After heat-killing restriction enzymes, add dpni to rid of background plasmids (circular template plasmids that are supposed to be linearized) - dpni works in most buffers
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