"

From 2010.igem.org

REMEMBER TO BE STERILE

• Take 5mL LB and put in a round bottom tube
• If your antibiotic is 1000x (which it should be) add 5uL antibiotic to your tube

  *MAKE SURE YOU ADD THE RIGHT ONE!

• Take a tip on a p200 and lightly touch a colony
• Eject the tip into the culture and put the cap on
• For a miniprep, grow O/N for 12-16 hours NOT LONGER

[http://openwetware.org/wiki/Bacterial_cell_culture]