"
Week9 8/8/10-8/14/10
From 2010.igem.org
8/11/10
Digested and ran a gel of CHS3 and backbone plasmids (C,T,K,A)
Tested restriction enzymes by digesting circular plasmids with each enzyme individually --> should see 1 2500kb band
Organized the DNA samples in our -20.
8/12/10
Weekly meeting
- Went over our gels --> identified faulty parts (holin1, holin2, CHS3-pMal, CP-LacPI)
- Plan to re-kit to stock and reassemble
Decided to use an alternative stain because calcofluor results in too much background and our confocal microscope can not excite in the UV spectrum
8/13/10
Methanol fixation of yeast and E.Coli cells --> stained with rhodamine-conjugated chitin probe (allowed to incubate overnight)
Competency of ChiA knockouts = 2.5x10^7 and 5.0x10^6
Cycles 1 of File:MAGE for tqsA Knockouts.
