Team:Panama/24 August 2010
From 2010.igem.org
We performed the E. coli transformation, with plasmid (chloramphenicol resistance) + RBS + promoter. This time we used NEB protocol.
We made a miniprep and ran in agarose gel 1%.
1. Hind III marker (1 uL ladder, 2 uL loading, 7 uL H2O). 2. Sample (5 uL ligation + 2 uL loading).