Team:Yale/LabNotebook/Week1

From 2010.igem.org

Monday 6/7--defended project (yay!)

Wednesday 6/9--started in lab--got set up & started cultures of DH5alpha and LE392

Thursday 6/10--inoculated liquid cultures in AM, made up different copper sulfate & LB solutions, then ran first copper growth assay in plate reader in PM w/three different ODs of each strain(4 hr. protocol)

Friday 6/11--Ran another growth assay with middling concentrations based on results of first, but bacteria failed to grow well--b/c allowed to overgrow?

Monday 6/14--Primer design, redo of Friday's growth assay

Tuesday 6/15--more primer design & plasmid synthesis planning (see posted plan on google group), spotted cell soln's from Monday growth assay to see if bacteria survived

Wednesday 6/16--checked on spotted cell survival assay, collected MOPS minimal media materials, started making component solutions

Thursday 6/17--more minimal media work and meeting, started BL21 culture

Friday 6/18--plasmid pSB74 should arrive! Make chemically competent versions of all cell lines. Transform all cell lines with pSB74 and with promoter and terminator (taken from kit). Make up plates with antibiotics. Help would be welcome :)

Saturday 6/19--take plates w/ transformed cells out of incubator & leave in cold room

Sunday 6/20--in evening inoculate 5 mL liquid cultures