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Team:Harvard/allergy/allergens
From 2010.igem.org
meet the allergens
GFP (Green Fluorescent Protein)
GFP (green fluorescent protein) is a fluorescent protein that shows green florescence when exposed to blue light. Because of its fluorescent property, it is commonly used as a reporter. As a proof of principle we are knocking down GFP in a strain of Arabadopsis that is modified to express GFP.
LTP (Lipid Transfer Protein)
The Lipid transfer protein helps to transport lipids across cell membranes. Plant lipid transfer proteins are pan allergens responsible for allergies to a wide range of foods such as broccoli, carrots, celery, tomatoes, melons and kiwis. The extent of this protein's cross-reactivity is comparable to that of profilins. LTP is a particularly severe allergen because it is resistant to degradation by pepsin (enzyme used by the stomach to break down proteins).
Bet versions 1.1 and 1.2 (birch pollen analog)
Bet v 1 and Bet v 2 are analogs of a birch pollen protein that is, along with other plant pollen proteins, responsible for allergies in 100 million individuals worldwide. Bet v 1 is a previously identified allergen whose homologues have been found in hazel, hornbeam, and adder trees, as well as fruits such as apples, cherries, kiwis, and celery. Bet v 2 is a homologue of Bet v 1 found in Arabadopsis thaliana that we found specifically for the purpose of this summer's project.
At the molecular level, homologues of Bet v 1 share 65% to 56% homology. The structure of Bet v 1 and its homologues have an alpha helix that is surrounded by seven beta sheets that terminate in a large fork formed by two more consecutive alpha helixes. This forms a forked solvent-accessible cavity that transverses the entire molecule. See image here: http://btcpxx.che.uni-bayreuth.de/Fachgruppe_Chemie/grafik/Roesch_betv1_h.jpg
While the biological function of Bet v 1 and Bet v 2 are not entirely clear, the protein is expressed in plants during periods of stress
Ger version 3
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