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Contents 1 2010/10/09 1.1 1:PCR 1.2 2:Electrophoresis① 1.3 3:Electrophoresis② 1.4 4:Electrophoresis③ 1.5 5:Electrophoresis④ 1.6 6：DNA Digestion

2010/10/09

1:PCR

<Member>
Hitomi

<Sample>(temperature in annealing:℃)

• 1
• 2
• 3
• 4
• 5
• 6
• 7
• 8
• 9
• 10
• 1-2
• 2-3
• 2-7
• 2-10
• 4-5
• 6-3
• 6-7
• 7-8-10

<Protocol>
See Protocol 1

2:Electrophoresis①

<Member>
Hitomi

<Sample>
PCR products

• 10-2

<Protocol>
See Protocol 8

<Result>

3:Electrophoresis②

<Member>
Hitomi

<Sample>
PCR products (10/8)

<Protocol>
See Protocol 8

<Result>

4:Electrophoresis③

<Member>
Hitomi

<Sample>
PCR products (10/9)

<Protocol>
See Protocol 8

<Result>

5:Electrophoresis④

<Member>
Mariko

<Sample>
PCR products

• 1
• 2
• 3
• 4
• 5
• 6
• 7
• 8
• 9
• 10
• 2-10
• 4-5

<Protocol>
See Protocol 8

<Result>

6：DNA Digestion

＜Member＞
Mariko

＜Sample, Materials＞
・PCR productions

• 1
• 2
• 3
• 4
• 6
• 10
• 7-8-10
• vector

・Digest enzyme

• Avr2
• Nhe1
• Spe1

＜Protocol＞
See Prptpcpl 9