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Team:Cambridge/Gibson/Protocol
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Revision as of 09:56, 30 September 2010 by
Willh
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Introduction
Home
Meet the Team
Photo Gallery
Videos
Parts submitted
Project Firefly
Introduction
Background
Coloured outputs
Luciferin recovery
Modelling
Characterisation
Project Vibrio
Introduction
Background
The LuxBrick
Codon optimisation
Modelling
Characterisation
Tools
Gibthon
BioBrick → GenBank
Ligation Calculator
The E.glometer
Gibson Assembly
Introduction
How it works
Protocol
RFC
Considerations
Human Practices
Safety
Future applications
Biosensors
Lighting
Notebook
Summary
Week 1
Week 2
Week 3
Week 4
Week 5
Week 6
Week 7
Week 8
Week 9
Week 10
Week 11
Week 12
Beyond Week 12
Protocols
Our Partners
Sponsors
iGEM Teams
University
Publicity
Gibson Assembly: Protocols
Master mix
Volume/µl
Taq ligase (40u/µl)
50
5x isothermal buffer
100
T5 exonuclease (1u/µl)
2
Phusion polymerase (2u/µl)
6.25
Nuclease-free water
216.75
=375