Team:UCL London/Week 5

From 2010.igem.org

Revision as of 10:39, 29 September 2010 by Zcbee12 (Talk | contribs)

UCL IGEM 2010

RETURN TO IGEM 2010

Preparation week - Week 5

Monday 26th July

We started transformation of the DNA we need from the iGEM registry plates today. WE had 11 parts to transform, so Xiang and the team had quit a tough few days ahead of them.



UCL London test-tubes.jpg

 

Tuesday 27th July

UCL London test-tubes.jpg

The results of the transformation on the previous day were confirmedd today.


 

Wednesday 28th July

UCL-Gel.jpg
 

Thursday 29th July

UCL-meetingportico.JPG

This Thursday meeting started as a one time event..and became like a bad habit..but in a good way. The portico seams to provide a very inspiring atmosphere for the iGEM UCL members to discuss their weekly progress and the tasks assigned to each one. For once more, a lot of ideas regarding the project were circulated.


 

Friday 30th July

UCL London test-tubes.jpg

In terms of lab work, Currently, we have the following parts (confirmed by gel electrophoresis):

Inducible promoter lacI

Possible hypoxia promoter from UCL iGEM 2009 mNarK. Positive feedback loop promoter, LasR regulated. Terminator. Kanamycin backbone for assembly. Chloramphenicol backbone for assembly. Amp&Kana backbone as back-up plan for assembly. Amp&Chlor backbone as back-up plan for assembly.


The missing important parts we need for ligation

RBS + coding region of LasR + terminator Red dye pathway (or any other colour) Native hypoxia promoter NarK.

 

Saturday

Sunday

 

Retrieved from "http://2010.igem.org/Team:UCL_London/Week_5"