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From 2010.igem.org

Contents 1 1:PCR 2 2：DNA Digestion 3 3:Electrophoresis① 4 4:Electrophoresis② 5 5：DNA extraction from gels 6 6：DNA Ligation

1:PCR

<Member>
mio

<Sample>
(temperature in annealing)

• 3
• 6
• 8

<Protocol>
See Protocol 1

2：DNA Digestion

＜Member＞
mizuki

＜Sample, Materials＞
・PCR productions

• 1 (8/31)
• 2 (9/6)
• 4 (8/31)
• 5L (8/26)
• 7 (8/31)
• 8 (8/31)

・Digest enzyme

• 1,4,8 : AvrⅡ
• 2,7 : NheⅠ
• 5L : SpeⅠ

＜Protocol＞
See Prptpcpl 9

3:Electrophoresis①

<Member>
mio

<Sample>
PCR products (9/17)

• 3
• 6
• 8

<Protocol>
See Protocol 8

<Result>

4:Electrophoresis②

<Member>
mizuki

<Sample>
Digestion products (9/17)

• 1
• 2
• 4
• 5
• 7
• 8

<Protocol>
See Protocol 8

<Result>

5：DNA extraction from gels

＜Member＞
mizuki

＜Sample＞
Digestion products(9/17)

• 4
• 7
• 8

(After electrophoresis)

＜Protocol＞
See Protocol 4

6：DNA Ligation

＜Member＞
mizuki

＜Sample＞

• 7(9/17)
• 8(9/17)

(after digestion)

＜Protocol＞
See Protocol 10