From 2010.igem.org

Evaluation for wet laboratory

Goals of the evaluation

To determine the best possible parts for the biological implementation of E. lemming, the combined molecular model (Light switch - Chemotaxis) has been used to answer specific questions:

1. Which receptor (Che) protein should be attacked?
2. To which light-sensitive protein (PhyB, PIF3) should the receptor protein be linked?
3. What is a good aspartate concentration?
4. In what quantity should the anchor and binding light-sensitive protein be chosen?

Answers to this questions were able to decrease the experimental expenses significantly.

Evaluator

Combined models. Coupled individual models for the simulation of the whole process and their interfaces. The concentration of CheYp determines the movement bias.

Evaluation of the results is a general optimization problem. The output variable in the case of E. lemming is the concentration of free and phosphorylated CheYp, because it is directly linked to the movement bias. To achieve a reasonable evaluation variable, the relative amplitude of free and phosphorylated CheYp was chosen to be maximized.

Determination of relative amplitude. Relative amplitude of free CheYp was maximized to achieve to determine optimal parts.

Results

Evaluation for information processing

Goal of the evaluation

Main goal of the evaluation for information processing was to determine time constants of E. lemming by using the combined molecular model (Light switch - Chemotaxis).