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From 2010.igem.org

Evelyn:

PCR: pduJK with the primer of pduJK_f1 and pduJK_r2.

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Knightmare:

Colony PCR: pduAB in old standard.
Succeed and send for sequencing

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daodao:

Restriction Enzyme Cutting of pduTUV and PSB1C3 with the mutation

Concentration determination of product of Restriction Enzyme Cutting of pduTUV and PSB1C3 with the mutation

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gpw：

PCR:pduJK with the primer of pduJK_f3 and pduJK_r4

Concentration determination of product of pduJK 1-2，3-4，5-6-PCR

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