Determining how much vector, insert, and milliQ water should be used
Measure concentrations of the vector and insert samples to be used via nanodrop, spectrophotometer, etc.
The vector volume = [desired vector mass (ng)]/[vector concentration]
The insert volume = [(desired vector mass (ng))/(vector length)]*[(insert length)/(insert concentration)]*[desired insert:vector ratio]
milliQ water will be used as a filler to ensure that the end reaction volume is 20μL. This means milliQ volume = 20μL - 2μL (from DNA ligase buffer) - 1μL (from DNA ligase) - vector volume - insert volume.
We would like to take a moment to thank all of our sponsors for their very generous donations, as we could not have done this without your help!
We would also like to thank and acknowledge: Our Advisors
Marc Facciotti
Ilias Tagkopoulos Technical Guidance
David Larsen
Andrew Yao Visiting iGEMer
Jia Li of Zhejiang University (TEAM ZJU-China) cI Promoter Screen
Drew Endy - Stanford
Thomas Schneider - NIH
Want to sponsor us? Send an email to mtfacciotti@ucdavis.edu to discuss various ways you can help! :)
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