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Team:HokkaidoU Japan/NotebookTest
From 2010.igem.org
since 13 Jul 2010
since 1 Aug 2010
Calendar
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Abstract
Monday, July 12, 2010
- The first experiment for us, transformation with BioBrick Devices.
Wednesday, July 21, 2010
- Preparation of competent cells
- Single colony isolation of E. coli which had been transformed on Monday, July 12, 2010
Monday, July 26, 2010
- Cultivation of transformed E. coli for the next day's miniprep
Tuesday, July 27, 2010
- Miniprep
Monday, August 2, 2010
- Restriction enzyme digestion of plasmids which had been purified by miniprep
- Electrophoresis assay
Tuesday, August 3, 2010
Wednesday, August 4, 2010
Thursday, August 5, 2010
Friday, August 6, 2010
Monday, August 9, 2010
- Initial amplification of the BioBrick parts (BBa_I14032 "2-11P", BBa_F2621 "2-21H", BBa_K098995 "3-1E" and BBa_E1010 "1-18F") that are used in the construction of concentration-sensitive and heat-sensitive E. coli
- This is a preliminary experiment before starting the main project
Tuesday, August 10, 2010
- PCR amplification of the other parts (BBa_F1610 "2-24G", BBa_B0034 "1-2M", BBa_B0015 "1-23L" and BBa_K098995 "3-1E") and electrophoresis for confirmation
Wednesday, August 11, 2010
- Preparation for the next day's miniprep
- Estimation of enzyme activity
- Preparation of the glycerol stocks
Thursday, August 12, 2010
- Miniprep (1-18F, 2-21H and 2-11P)
- Electrophoresis of the DNA which had amplified via PCR and miniprep
Friday, August 13, 2010
- PCR amplification of the DNA that we couldn't obtain via miniprep previous day(1-18F)
Monday, August 16, 2010
- Measurement of restriction enzyme activity
- Restriction enzyme digestion of the parts(3-1E, 1-2M, 1-18F and 1-23L) and the vector(pSB1C3)
Tuesday, August 17, 2010
- Purification of the DNA solutions via gel extraction
- Preparation of agar medium containing 35 ug/mL chloramphenicol
