Acknowledgements

Heng H. Khun for the considerable amount of time you spent with us helping us with our wetlab portion of our project especially with lab techniques.

Deirdre Lobb for helping us with virtually everything from wetlab troubleshooting to providing us with all the materials we needed. This year’s iGEM team would not have been possible without your contributions.

Dr. Shawn Lewenza for your kind donation of periplasmic RFP strains as well as the digital sequence. This is a crucial piece in our transcription/translation protein folding plasmid.

Dr. Tracy Ravio for your contributions of culture with NlpE and CpxP plasmids. This part is significant to test protein aggregation in the periplasmic space.

Dr. Jean Michael Betton for providing us with essential genes MalE, MalE31, MalE31 with signal sequence deletion and MalE signal sequence deletion. This part will help us characterize protein aggregation in both cytoplasmic and periplasmic regions.

We would like to thank Dr. Lesli Nagata and Dr. John Wu as well as their colleagues for their roles in organizing a tour of the Defence Research and Development Canada base in Suffield. The discussions and the tour were very helpful in developing the Ethics component of our project.

Thane Kubik for lending us your expertise and experiences with iGEM, your contributions really helped us with troubleshooting our project.

Thanks to Sonia Georgijevic, Mandy Cheung, and Stefan Marcus for taking time out of your schedules to do an iGEM crash course for us. This helped get all of us into an iGEM mindset.

Leanne Yohemas for taking the time to meet with us regarding media coverage for our iGEM team.

Jennifer Hill from Alberta Innovates for organizing the iGEM teacher’s workshop in Calgary as well as setting up the Lethbridge workshop. Future thanks for the aGEM competition. All these events that you helped us with really helps facilitate a stronger iGEM community in Alberta.

Jennifer Logan and Toby Taylor

for all the administration help. iGEM could not be possible without your contributions.

Thank you to the University of Calgary BHSc faculty for allowing us to operate within your teaching labs. Without proper lab space, equipment and supplies, it would not have been possible to have an iGEM team this year.