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Team:TU Delft/17 June 2010 content
From 2010.igem.org
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Today camera crew #2 came by from Nanopodium, a platform for exchanging thoughts, ideas, opinions and best practices on nanotechnology. They are making a movie about our project for the Nano Video Hunt. This is a campaign started with Hyves were young people are challenged to post videos about their views on nanotechnology.
For more information look at the following websites:
[http://www.nanovideohunt.nl www.nanovideohunt.nl]
[http://www.nanovideohunt.hyves.nl www.nanovideohunt.hyves.nl]
[http://www.nanopodium.nl www.nanopodium.nl]
Lab work
By Hugo
I made some time for doing some transformations from the Biobrick plates, I will give you the list of the biobricks that I tried to transform.
| # | Code | Resistance marker | Function |
| 1 | J61100 | Amp | RBS Anderson family |
| 2 | J61101 | Amp | RBS Anderson family |
| 3 | J61107 | Amp | RBS Anderson family |
| 4 | J61117 | Amp | RBS Anderson family |
| 5 | J61127 | Amp | RBS Anderson family |
| 6 | B0034 | Amp | RBS |
| 7 | J23109 | Amp | Promoter Anderson family |
| 8 | B0032 | Amp | RBS Anderson family |
| 9 | R0011 | Amp | LacI promoter |
| 10 | B0015 | Amp, Kan | Double terminator |
| 11 | R0010 | Amp | LacI |
| 12 | E0422 | Amp | YFP generator |
| 13 | E0240 | Amp | GFP generator |
| 14 | E0040 | Amp | GFP |
| 15 | I13401 | Amp | GFP + Double terminator |
Procedure:
- Add 1 ul of DNA to 33 ul of competent cells
- Put the tubes on ice for 10 min
- Warm the tubes in a water bath at 37º C for 5 min.
- Add 250 ul of LB medium
- Incubate the cells 1 hour at 37ºC, shake at 160 rpm
- Plate 200 ul over selective medium
