Team:Kyoto/Notebook

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Contents

Index

Notebook

001: Preparing

  • By: Wataru, Tomo, Yuki, Kazuya, Ken, Makoto
  • Date: 07/20
  • Category: Antibiotic, Parts, Transformation
  • Protocol: Use BioBrick Parts

1. Solubilization fo antibiotics.

  1. Make two mixtures as the following.
    • 1g Ampicillin and 20ml MilliQ (Final concentration 50mg/ml).
    • 0,5g Ampicillin and 10ml MilliQ (Final concentration 50mg/ml).
  2. Dispense 1.1ml to 1.5ml tubes.
  3. Keep in -20℃ Freezer.

2. Make LB plate.

9 plates for Ampicillin+ and Kanamycin+.

3. Transformation of iGEM Parts.

NameWell*1Sample (µl)Competent Cells (µl)Total (µl)PlateIncubationResult
<partinfo>J23100</partinfo>1-18-C12021LB (Ampicillin+)At 37℃ 7/20 20:50 - 7/21 17:00
<partinfo>J23105</partinfo>1-18-M12021
<partinfo>J23116</partinfo>1-20-M12021
<partinfo>R0011</partinfo>1-6-G12021
<partinfo>E0840</partinfo>1-12-O12021
<partinfo>J06702</partinfo>2-8-E12021
<partinfo>pSB4K5</partinfo>1-5-G12021×
<partinfo>B0015</partinfo>1-23-L12021LB (Kanamycin+)×
  • *1 "1-18-C" means well 18C in [http://partsregistry.org/Help:Spring_2010_DNA_distribution Spring 2010 DNA Distribution Kit] Plate 1.

Discussion

A vector of "pSB4K5" is Kanamycin-resistance, however, we plated it to LB plate (Ampicillin+).

And We didn't pre-culture "B0015" despite its vector is Kanamycin-resistance.

So, it was predicted that we will fail the transformation of "pSB4K5" and "B0015".