| Biobrick name |
Description |
Creation date |
Strain |
Vector |
Quality control |
Length |
Store |
|
<partinfo>BBa_E2050</partinfo>
|
mOrange
|
08/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB2K3</partinfo>
|
Seq ok (MIT); orange pellet when contained in <partinfo>pSB2K3</partinfo>
|
744 bp | BioBricks 2010 |
|
<partinfo>BBa_K165018</partinfo>
|
NLS-ADH1 terminator (Silver Standard 5' and 3')
|
08/06/2010
|
E. coli DH5-alpha
|
<partinfo>J63009</partinfo> (Amp)
|
Seq partially confirmed (MIT) because it lacks a T at the 3', but the ADH1 terminator (i.e. <partinfo>BBa_K165010</partinfo>) is ok. The correct ApE file is here.
|
253 bp | BioBricks 2010 |
|
<partinfo>BBa_K165037</partinfo>
|
tef2 promoter
|
08/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1AK3</partinfo>
|
Seq ok (MIT).
|
403 bp | BioBricks 2010 |
|
<partinfo>BBa_P1004</partinfo>
|
Chloramphenicol resistence
cassette
|
08/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1A1</partinfo>
|
Seq ok (MIT 2009); length ok.
|
769 bp | BioBricks 2010 |
|
<partinfo>BBa_J61001</partinfo>
|
R6K origin
|
08/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1A2</partinfo>
|
Seq ok (MIT).
|
406 bp | BioBricks 2010 |
|
<partinfo>BBa_K081008</partinfo>
|
RBS-luxI
|
08/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1A2</partinfo>
|
Seq ok (MIT); length ok.
|
664 bp | BioBricks 2010 |
|
<partinfo>BBa_K125500</partinfo>
|
GFP fusion brick (Silver Standard 5', Knight Standard 3')
|
08/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1A2</partinfo>
|
Seq partially confirmed (MIT) for unknown reasons: the alignment is fully correct. The ApE file with the correct prefix and suffix is here. Length ok.
|
718 bp | BioBricks 2010 |
|
<partinfo>BBa_K208001</partinfo>
(stab-1)
|
phasin phaP
(Silver Standard 5' and 3') |
24/06/2010
|
E. coli ???
|
<partinfo>pSB3K3</partinfo>
|
Seq ok (BMR); length ok.
|
579 bp | BioBricks 2010 |
|
<partinfo>BBa_K208001</partinfo>
(stab-2)
|
phasin phaP
(Silver Standard 5' and 3') |
24/06/2010
|
E. coli ???
|
<partinfo>pSB3K3</partinfo>
|
Seq ok (BMR); length ok.
|
579 bp | BioBricks 2010 |
|
<partinfo>BBa_J72007</partinfo>
|
BamHI methyltransferase encoding CRIM plasmid
|
01/07/2010
|
E. coli ???
|
HC Cm resistance
|
No plasmid in the clone.
For more informations click here
|
| BioBricks 2010 |
|
<partinfo>BBa_J72008</partinfo>
|
phi80 integration helper plasmid pInt80-649
|
01/07/2010
|
E. coli ???
|
Plasmid (Amp 50 ug/ml resistance)
|
Length ok.
|
6335 bp | BioBricks 2010 |
|
<partinfo>BBa_J72013</partinfo>
|
BglII methyltransferase encoding CRIM plasmid
|
01/07/2010
|
E. coli ???
|
HC Cm resistance
|
No plasmid in the clone.
For more informations click here
|
|
BioBricks 2010 |
|
<partinfo>BBa_K105012</partinfo>
|
10 aa flexible protein domain linker
|
06/08/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1A2</partinfo>
|
-
|
30 bp |
BioBricks 2010 |
|
<partinfo>BBa_J13002</partinfo>
|
p(tetR)+RBS34
|
16/08/2010
|
E. coli TOP10
|
<partinfo>pSB1A2</partinfo>
|
-
|
74 bp |
BioBricks 2010 |
|
<partinfo>BBa_I13522</partinfo>
|
Untagged GFP behind a constitutive promoter
|
16/08/2010
|
E. coli TOP10
|
<partinfo>pSB1A2</partinfo>
|
-
|
937 bp |
BioBricks 2010 |
| |
| Ligation name |
Description |
Creation date |
Strain |
Vector |
Quality control |
Length |
Store |
|
I0
(it was I0-2)
|
<partinfo>BBa_K125500</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)
|
11/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1AK3</partinfo>
|
Seq ok (BMR); length ok.
|
853 bp | Ligations 2010 |
|
I1
(it was I1-2)
|
<partinfo>BBa_E2050</partinfo> (E-S)
+
<partinfo>BBa_K165018</partinfo> (E-X) |
11/06/2010
|
E. coli DH5-alpha
|
<partinfo>J63009</partinfo> (Amp)
|
Seq ok (BMR), with an additional barcode (25bp) after the stop codon of mOrange; length ok.
|
1029 bp |
Ligations 2010 |
|
I2
(it was I2-1)
|
<partinfo>BBa_P1004</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)
|
11/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1AK3</partinfo>
|
Seq ok (BMR); Cm resistance ok; length ok.
|
906 bp | Ligations 2010 |
|
I3
(it was I3-1)
|
<partinfo>BBa_K081008</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X) |
11/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1AK3</partinfo>
|
Seq TWO PLASMIDS IN THIS CLONE!(BMR), two overlapped traces; length ok.
|
801 bp | Ligations 2010 |
|
I4
(it was I4-2)
|
<partinfo>BBa_K165037</partinfo> (S-P)
+
I1 (X-P) |
18/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1AK3</partinfo>
|
Seq ok (BMR); length ok.
|
1439 bp |
Ligations 2010 |
|
I5
(it was I5-1)
|
I2 (E-S)
+
<partinfo>BBa_J61001</partinfo> (E-X) |
18/06/2010
|
E. coli TOP10
|
<partinfo>pSB1A2</partinfo>
|
Seq ok (BMR); Cm resistance ok; length ok.
|
1320 bp | Ligations 2010 |
|
I6
(it was I6-2)
|
I3 (E-S)
+
<partinfo>BBa_T9002</partinfo> (E-X) |
18/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1A3</partinfo>
|
Seq LONG PART(BMR), but the ligation worked and the sequenced portions are ok; length ok.
|
2754 bp |
Ligations 2010 |
|
I7
(it was I7-3)
|
<partinfo>BBa_J23118</partinfo> (E-S)
+
I6 (X-P) |
25/06/2010
|
E. coli TOP10
|
<partinfo>BBa_J61002</partinfo>
(Amp)
|
Length ok.
|
2795 bp |
Ligations 2010 |
|
I8
(it was I8-5)
|
<partinfo>BBa_J23110</partinfo> (E-S)
+
I6 (X-P) |
25/06/2010
|
E. coli TOP10
|
<partinfo>BBa_J61002</partinfo>
(Amp)
|
Length ok.
|
2795 bp |
Ligations 2010 |
|
I9
(it was I9-1)
|
<partinfo>BBa_J23111</partinfo> (E-S)
+
I6 (X-P) |
25/06/2010
|
E. coli TOP10
|
<partinfo>BBa_J61002</partinfo>
(Amp)
|
Length ok.
|
2795 bp |
Ligations 2010 |
|
I10
(it was I10-1)
|
<partinfo>BBa_J23116</partinfo> (E-S)
+
I6 (X-P) |
25/06/2010
|
E. coli TOP10
|
<partinfo>BBa_J61002</partinfo>
(Amp)
|
Length ok.
|
2795 bp |
Ligations 2010 |
|
I12
(it was I12-2)
|
<partinfo>BBa_J23105</partinfo> (S-P)
+
I6 (X-P)
|
16/07/2010
|
E. coli TOP10
|
<partinfo>BBa_J61002</partinfo>
(Amp)
|
Seq ok (BMR), length ok.
|
2795 bp |
Ligations 2010 |
|
I14
(it was I14-1)
|
<partinfo>BBa_J23106</partinfo> (S-P)
+
I3 (X-P)
|
16/07/2010
|
E. coli DH5-alpha
|
<partinfo>BBa_J61002</partinfo>
(Amp)
|
Seq ok (BMR), length ok.
|
842 bp |
Ligations 2010 |
|
RING
|
I5 (X-S):
Cm resistance (<partinfo>BBa_P1004</partinfo>) + TT (<partinfo>BBa_B0015</partinfo>) + R6K ori (<partinfo>BBa_J61001</partinfo>)
|
16/07/2010
|
E. coli DH5-alpha
|
Plasmid (Cm resistance, conditional thermosensitive ORI; works in PIR strains)
|
Tested here.
|
1328 bp
|
Ligations 2010 |
|
I15
(it was I15-1)
|
<partinfo>BBa_J23110</partinfo> (S-P)
+
I3 (X-P)
|
23/07/2010
|
E. coli DH5-alpha
|
<partinfo>BBa_J61002</partinfo>
(Amp)
|
Length ok.
|
842 bp |
Ligations 2010 |
|
I16
(it was I16-1)
|
<partinfo>BBa_J23116</partinfo> (S-P)
+
I3 (X-P)
|
16/07/2010
|
E. coli DH5-alpha
|
<partinfo>BBa_J61002</partinfo>
(Amp)
|
Seq ok (BMR), length ok.
|
842 bp |
Ligations 2010 |
|
I17
(it was I17-1)
|
<partinfo>BBa_J23101</partinfo> (S-P)
+
I3 (X-P)
|
16/07/2010
|
E. coli DH5-alpha
|
<partinfo>BBa_J61002</partinfo>
(Amp)
|
Seq ok (BMR), length ok.
|
842 bp |
Ligations 2010 |
|
I18
(it was I18-1)
|
<partinfo>BBa_J23105</partinfo> (S-P)
+
I3 (X-P)
|
16/07/2010
|
E. coli DH5-alpha
|
<partinfo>BBa_J61002</partinfo>
(Amp)
|
Seq ok (BMR), length ok.
|
842 bp |
Ligations 2010 |
|
I19
(it was I19-1)
|
<partinfo>BBa_J23106</partinfo> (S-P)
+
I3 (X-P)
|
16/07/2010
|
E. coli DH5-alpha
|
<partinfo>BBa_J61002</partinfo>
(Amp)
|
Seq ok (BMR), length ok.
|
842 bp |
Ligations 2010 |
|
I20
(it was I20M-1)
|
Pha-10S-1 (X-S) (infos here)
+
<partinfo>pSB1AK3</partinfo> (X-S)
|
13/08/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1AK3</partinfo>
|
Length ok.
|
576 bp
|
Ligations 2010 |
|
I21
(it was I21-4)
|
Pha-SS-1 (X-S) (infos here)
+
<partinfo>pSB1A3</partinfo> (X-S)
|
10/08/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1A3</partinfo>
|
Length ok.
|
576 bp
|
Ligations 2010 |
|
I22
(it was I22-1)
|
<partinfo>BBa_K081009</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)
|
10/08/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1AK3</partinfo>
|
Length ok.
|
825 bp
|
Ligations 2010 |
|
I23
(it was I23-1)
|
<partinfo>BBa_R0062</partinfo> (S-P)
+
I3 (X-P)
|
10/08/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1AK3</partinfo>
|
Length ok.
|
725 bp
|
Ligations 2010 |
|
I24
(it was I24-1)
|
<partinfo>BBa_R0062</partinfo> (S-P)
+
I22 (X-P)
|
13/08/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1AK3</partinfo>
|
Length ok.
|
886 bp
|
Ligations 2010 |
|
I26
(it was I26-2)
|
<partinfo>BBa_K105012</partinfo> (S-P)
+
I21-B (X-P)
|
13/08/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1A2</partinfo>
|
Length ok.
|
612 bp
|
Ligations 2010 |
|
I29
(it was ??)
|
<partinfo>BBa_T9002</partinfo> (S-P)
+
I23 (X-P)
|
|
E. coli TOP10
|
<partinfo>pSB1A3</partinfo>
|
|
725 bp
|
Ligations 2010 |
|
I30
(it was ??)
|
<partinfo>BBa_T9002</partinfo> (S-P)
+
I24 (X-P)
|
|
E. coli TOP10
|
<partinfo>pSB1A3</partinfo>
|
|
886 bp
|
Ligations 2010 |
|
I31
(it was I31-1)
|
I20 (E-S)
+
<partinfo>BBa_K105012</partinfo> (E-X)
|
23/08/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1A2</partinfo>
|
Length ok.
|
614 bp
|
Ligations 2010 |
|
I32
(it was ??)
|
I20 (E-S)
+
I21 (E-X)
|
|
E. coli DH5-alpha
|
<partinfo>pSB1A3</partinfo>
|
|
1158 bp
|
Ligations 2010 |
|
I33
(it was I33-1)
|
I20 (E-S)
+
I0 (E-X)
|
23/08/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1AK3</partinfo>
|
Length ok.
|
1437 bp
|
Ligations 2010 |
|
I34
(it was I34-1)
|
I21 (E-S)
+
I0 (E-X)
|
23/08/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1AK3</partinfo>
|
Length ok.
|
1437 bp
|
Ligations 2010 |
|
I35
(it was I35-1)
|
I21 (E-S)
+
<partinfo>BBa_K105012</partinfo> (E-X)
|
23/08/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1A2</partinfo>
|
Length ok.
|
614 bp
|
Ligations 2010 |
|
I36
(it was ??)
|
I21 (E-S)
+
I21 (E-X)
|
|
E. coli DH5-alpha
|
<partinfo>pSB1A3</partinfo>
|
|
1158 bp
|
Ligations 2010 |
|
I37
(it was I37-1)
|
INTEIN (E-S)
+
I0 (E-X)
|
23/08/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1AK3</partinfo>
|
Length ok.
|
1366 bp
|
Ligations 2010 |
|
I38
(it was I38-3)
|
<partinfo>BBa_F2620</partinfo> (E-S)
+
<partinfo>BBa_B0034</partinfo> (E-X)
|
23/08/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1A2</partinfo>
|
Length ok.
|
1079 bp
|
Ligations 2010 |
|
I39
(it was ??)
|
<partinfo>BBa_J13002</partinfo> (S-P)
+
I20 (X-P)
|
|
E. coli DH5-alpha
|
<partinfo>pSB1AK3</partinfo>
|
|
652 bp
|
Ligations 2010 |
|
I40
(it was ??)
|
I26 (E-S)
+
INTEIN (E-X)
|
|
E. coli DH5-alpha
|
pMA (HC Amp resistance)
|
|
1125 bp
|
Ligations 2010 |
|
I41
(it was I41-1)
|
I26 (E-S)
+
I0 (E-X)
|
23/08/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1AK3</partinfo>
|
Length ok.
|
1471 bp
|
Ligations 2010 |
|
I42
|
MyCRIM (E-P)
+
A2 (E-P)
|
23/08/2010
|
E. coli' BW23474
|
<partinfo>MyCRIM</partinfo>
|
Length ok.
|
?? bp
|
Ligations 2010 |
|
I43
|
MyCRIM (E-P)
+
J101 (E-P)
|
23/08/2010
|
E. coli' BW23474
|
<partinfo>MyCRIM</partinfo>
|
Length ok.
|
?? bp
|
Ligations 2010 |
|
I44
|
MyCRIM (E-P)
+
Inserto di 4C5 (gene della morte) (E-P)
|
23/08/2010
|
E. coli' DB3.1
|
<partinfo>MyCRIM</partinfo>
|
Length ok.
|
?? bp
|
Ligations 2010 |
|
I45
|
MyCRIM (HindIII) su se stesso
|
23/08/2010
|
E. coli' BW23474
|
<partinfo>MyCRIM</partinfo>
|
Length ok.
|
?? bp
|
Ligations 2010 |
|
INTEIN
MR.G
|
Intein
|
13/08/2010
|
E. coli TOP10
|
pMA
(HC Amp resistance)
|
Mr.Gene DNA synthesis
|
507 bp
|
Ligations 2010 |
|
CRIM
MR.G
|
CREAM fragment
|
13/08/2010
|
E. coli TOP10
|
pMK-RQ
(HC Kan resistance)
|
Mr.Gene DNA synthesis
|
829 bp
|
Ligations 2010 |
|
I7-4C5
(it was I7-4C5-2)
|
I7 (E-P)
+
<partinfo>pSB4C5</partinfo>(E-P)
|
16/07/2010
|
E. coli TOP10
|
<partinfo>pSB4C5</partinfo>
|
Seq ok (BMR), length ok.
|
2795 bp |
Ligations 2010 |
|
I8-4C5
(it was I8-4C5-2)
|
I8 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)
|
16/07/2010
|
E. coli TOP10
|
<partinfo>pSB4C5</partinfo>
|
Seq ok (BMR), length ok.
|
2795 bp |
Ligations 2010 |
|
I10-4C5
(it was I10-4C5-1)
|
I10 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)
|
16/07/2010
|
E. coli TOP10
|
<partinfo>pSB4C5</partinfo>
|
Length ok.
|
2795 bp
|
Ligations 2010 |
|
I12-4C5
(it was I12-4C5-1)
|
I12 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)
|
25/07/2010
|
E. coli TOP10
|
<partinfo>pSB4C5</partinfo>
|
Length ok.
|
2795 bp
|
Ligations 2010 |
|
I14-4C5
(it was I14-4C5-2)
|
I14 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)
|
23/07/2010
|
E. coli DH5-alpha
|
<partinfo>pSB4C5</partinfo>
|
Length ok.
|
842 bp
|
Ligations 2010 |
|
I15-4C5
(it was I15-4C5-2)
|
I15 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)
|
13/08/2010
|
E. coli TOP10
|
<partinfo>pSB4C5</partinfo>
|
Length ok.
|
842 bp
|
Ligations 2010 |
|
I16-4C5
(it was I16-4C5-1)
|
I16 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)
|
23/07/2010
|
E. coli DH5-alpha
|
<partinfo>pSB4C5</partinfo>
|
Length ok.
|
842 bp
|
Ligations 2010 |
|
I17-4C5
(it was I17-4C5-1)
|
I17 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)
|
25/07/2010
|
E. coli DH5-alpha
|
<partinfo>pSB4C5</partinfo>
|
Length ok.
|
842 bp
|
Ligations 2010 |
|
I18-4C5
(it was I18-4C5-1)
|
I18 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)
|
25/07/2010
|
E. coli DH5-alpha
|
<partinfo>pSB4C5</partinfo>
|
Length ok.
|
842 bp
|
Ligations 2010 |
|
I19-4C5
(it was I19-4C5-1)
|
I19 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)
|
25/07/2010
|
E. coli DH5-alpha
|
<partinfo>pSB4C5</partinfo>
|
Length ok.
|
842 bp
|
Ligations 2010 |
|
I27
(it was ??)
|
I23 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)
|
??
|
E. coli TOP10
|
<partinfo>pSB4C5</partinfo>
|
|
725 bp
|
Ligations 2010 |
|
I28
(it was ??)
|
I24 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)
|
??
|
E. coli TOP10
|
<partinfo>pSB4C5</partinfo>
|
|
886 bp
|
Ligations 2010 |
| |
| Strain name |
Description |
Arrival date |
Infos |
Resistance |
Quality control |
Notes |
Store |
|
MG1655(Seq)
(MG)
|
E. coli wild type strain fully sequenced
|
28/06/2010
|
link
|
- |
|
Genome fully sequenced |
Strains 2010 |
|
MC1061
(MC)
|
E. coli strain
|
28/06/2010
|
link
|
- |
|
Strain used by JC Anderson et al. for integration |
Strains 2010 |
|
BW25141
(BW1)
|
E. coli pir+ strain
|
28/06/2010
|
link
|
- |
Actually replicates the conditional replication origin R6K |
LC pir |
Strains 2010 |
|
BW25142
(BW2)
|
E. coli pir116 strain
|
28/06/2010
|
link
|
- |
Actually replicates the conditional replication origin R6K |
HC pir |
Strains 2010 |
|
BW23473
|
E. coli pir+ strain
|
19/07/2010
|
link
|
- |
|
LC pir |
Strains 2010 |
|
BW23474
|
E. coli pir116 strain
|
28/06/2010
|
link
|
- |
Actually replicates the conditional replication origin R6K |
HC pir |
Strains 2010 |
|
BW5328/pAH123
|
E. coli strain
|
28/06/2010
|
link
|
Amp 50 ug/ml |
Plasmid length ok (after digestion with SpeI). |
Contains pAH123 HELPER plasmid carrying IntPhi80, low copy Amp, ori-ts 30°C
|
Strains 2010 |
|
BT340
|
E. coli strain
|
28/06/2010
|
link
|
Amp 100 ug/ml and Cm 34 ug/ml |
Plasmid relative molecular weight ok (after gel run of the undigested plasmid) |
Contains pCP20 helper with yeast FRT recombinase, Amp, Cm, ori-ts 30°C
|
Strains 2010 |
|
DSM15372
|
E. coli DMS15372 strain
|
08/07/2010
|
link
|
Amp 100 ug/ml |
Length ok (after digestion with EcoRI) |
Harbours the pBHR68 plasmid (contains an IPTG-inducible phaCAB operon)
|
Strains 2010 |
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