Aim 1: DNA reproduction+PCR
For PCR key see PCR key
Colourcode for the primers: Annealing part, mutagenised part, other sequences integrated into primer
PCR1: replication of the Tet-inducible CMV promotor [X]
Primer used: 1TreF 2TreR
expected product size: 492 bp
PCR4a: replication human bak with mutagenesis (Pst1) [ ]
Primer used: 7BakF 8BakMutPR
expected product size: 330 bp
PCR4b: replication human bak with mutagenesis (Pst1) [ ]
Primer used: 9BakMutPF 10BakR
expected product size: 376 bp
PCR5: joining PCR of human bak [ ]
Primer used: 7BakF 10BakR
expected product size: 688 bp
PCR6: replication of the SV40-polyadenylation site [X]
Primer used: 11PAF 12PAR
expected product size: 237 bp
PCR9: replication of eGFP with attP in primer [ ]
Primer used: 20eGFPattPF 21eGFPR
expected product size: 1213 bp
PCR10: Replikation of PhiC31o [ ]
Primer used: 22PC31oF 23PC31oR
expected product size: bp
Note: for attB two Primers ( 18attBF 19attBR) used.
Aim 2: Assembling Biobricks
Aim 3: Testing products
Aim 4: Testing system
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