Team:LMU-Munich/Schedule

PCR and mutagenisis of the digestion sites

PCR1a: replication of Pdu D with mutagenesis [ ] expected product size: bp

PCR1b: replication of Pdu D with mutagenesis [ ] expected product size: bp

PCR2: Joining PCR of PduD [ ] expected product size: bp

PCR3a: replication of Pdu C with mutagenesis [ ] expected product size: bp

PCR3b: replication of Pdu C with mutagenesis [ ] expected product size: bp

PCR4: Joining PCR of Pdu C [ ] expected product size: bp

PCR5: replication of AurF to be joined with Pdu D [ ] expected product size: bp

PCR6a: replication of LctO with mutagenesis [ ] expected product size: bp

PCR6b: replication of LctO with mutagenesis [ ] expected product size: bp

PCR7: Joining PCR of LctO [ ] expected product size: bp

PCR8a: replication of LctO with mutagenesis [ ] expected product size: bp

PCR8b: replication of LctO with mutagenesis [ ] expected product size: bp

PCR9: Joining PCR of LctO [ ] expected product size: bp

PCR10: Joining PCR of AurF and PduD [ ] expected product size: bp

PCR11: Joining PCR of LctO and PduC [ ] expected product size: bp

PCR12a: replication of the Pdu-Operon with mutagenesis [ ] expected product size: bp

PCR12b: replication of the Pdu-Operon with mutagenesis [ ] expected product size: bp

PCR13: Joining PCR of the Pdu-Operon [ ] expected product size: bp

PCR14a: replication of the Pdu-Operon with mutagenesis [ ] expected product size: bp

PCR14b: replication of the Pdu-Operon with mutagenesis [ ] expected product size: bp

PCR15: Joining PCR of the Pdu-Operon [ ] expected product size: bp

PCR16a: replication of the Pdu-Operon with mutagenesis [ ] expected product size: bp

PCR16b: replication of the Pdu-Operon with mutagenesis [ ] expected product size: bp

PCR17: Joining PCR of the Pdu-Operon expected product size: bp