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From 2010.igem.org

generation of subparts

subparts

	Protein	primers	PCR product	library of transformants	single colonies	storage vector	insert
che proteins	CheY-A	recieved	purified on stock	on agar plate	not available	purified on stock	on stock
	CheY-C	recieved	purified in stock	on agar plate	not available	purified on stock	on stock
	CheB-A	recieved	purified in stock	on agar plate	3 on agar plate	purified on stock	on stock
	CheB-C	recieved	purified in stock	on agar plate	3 on agar plate	purified on stock	on stock
	CheR-A	recieved	purified in stock	on agar plate	3 on agar plate	digest has to be redone
	CheR-C	recieved	purified in stock	on agar plate	3 on agar plate	digest has to be redone
localizers	TrigF-A	recieved	purified in stock	on agar plate	3 on agar plate	purified on stock
	MreB-C	recieved	purified in stock	in process
	tetR-A	recieved	purified in stock	on agar plate	3 on agar plate	purified on stock	on stock
couplers	PIF3-A	recieved	purified in stock	on agar plate	3 on agar plate	purified on stock
	PIF3-C	recieved	purified in stock	on agar plate	3 on agar plate	purified on stock
	PhyB-C	recieved	purified in stock	on agar plate	3 on agar plate	purified on stock
reporters	cyFP-A	recieved	purified in stock	on agar plate	3 on agar plate	purified on stock	on stock
	cyFP-C	recieved	purified in stock	on agar plate	3 on agar plate	purified on stock

generation of biobricks

testing of che-fusions

We're currently trying to establish an assay to test for the chemotactic behavour of E. coli. A first try with a "syringe test" failed. We'll redo the test and are looking for other possibilites. Maybe a swarm test?

testing of localicer-fusions

testing of couplers

testing of biobricks

bringing E. lemming alive

timetable