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From 2010.igem.org

Mimmi

LMWP C and N

Plasmid prep and glycerol stock

The cells were cultured to 0.6 OD, 1.6 ml were used to the glycerol stock and 10 ml were used to extract the plasmid

DNA concentrations

Sample	DNA (ng/μl)
LMWP N	60
LMWP C	80

over-expression in pEX - remaking the comassie-gel

MIX:

1. 4ml LB
2. 40µl glycose (1%)
3. 8µl Amp (0,5%)

Add a pipette tip of BL21 pEX.BBa_J18932 from glycerol stock and grow ON