Team:Stanford/Notebook/Timeline/Team Analog

From 2010.igem.org

Timeline for Team Analog

• Week 1: 6-28-2010
  • [x] Get parts and competent cells.
  • [x] Culture parts.
  • [x] Miniprep parts/make stocks.
  • [_] Characterize parts (promoters).

• Week 2: 7-05-2010
  • [x] Restriction digest parts.
  • [x] PCR superfolded GFP reporter.
  • [x] Gel extract parts.
  • [_] Order genes for kinases, phosphatases, and transcription factors.
  • [_] Characterize parts (promoters).

• Week 3: 7-12-2010
  • [x] Ligate parts.
  • [x] Order primers for small parts and promoters.
  • [x] Prepare MDV presentation and get money (hopefully).
  • [x] Order genes for kinases, phosphatases, and transcription factors.
  • [_] Streak BW27783 and culture to lose its current plasmid.
  • [_] Characterize parts (promoters).

• Week 4: 7-19-2010
  • [_] Receive primers and build promoters.
  • [_] Streak BW27783 and culture to lose its current plasmid.
  • [_] Transform ligations and conduct sequencing.
  • [_] Characterize parts (promoters).
  • [_] Try chromosomal integration proposed by Francois (for TF proomoter and reporter maybe?).
  • [_] Order more primers (degradation tags, truncated proteins, etc.).

• Week 5: 7-26-2010
  • [_] Receive protein parts and finish with assembly.
  • [_] Characterize protein parts (conduct kinetic assays, in vivo and in vitro).
  • [_] Do other promoter stuff (mutagenesis for better linearity, substrate specificity, attempt stoplight problem?)

• Week 6: 8-02-2010
  • [_] Improve design.

• Week 7: 8-09-2010
  • [_] Win iGEM. Already.

• Week 8: 8-16-2010

• Week 9: 8-23-2010

• Week 10: 7-30-2010
  • NOTE: Wednesday (8-1-2010). Housing contracts expire; move out by noon.