Team:TU Delft/protocols/ligation
From 2010.igem.org
Ligation
Ligations (pasting plasmid DNA) were performed at the appropriate temperature with the appropriate buffer in the appropriate concentration, according to the supplier.
Reaction for one sample:
DNA insert | x μL |
DNA vector | x μL |
T4 Ligation buffer (10×) | 2.0 μL (for 1×) |
T4 Ligase | 1.0 μL |
H2O | x μL |
20.0 μL (final contration ~20 ng/μL) |
Transform circa half of the ligation mix. Incubate at 16 °C o/n