Team:Tsinghua/Notebook/17 September 2010
From 2010.igem.org
Module Ⅱ, group 4
According to the known DNA sequence of OmpA and CBD, design the primer for the following Two-step PCR.
primer design:
OmpA
Upstream primer: TCATCATATGAAAGCTACTAAACTGGTACTGG Downstream primer: ACGCCAGTACCTGCAAGCTTTGAGCTCCCTCCTCCAGA
CBD
Upstream primer: ggagggagctcaAAGCTTGCAGGTACTGGCGTCGTATC Downstream primer: ACTGCTCGAGAGTTGAACCGTAAGCAAGAGTTCC
NOTE:the downstream primer of OmpA and the upstream primer of CBD will be matching during the PCR, so the Two-step PCR can be realized.
Order the designed preimers.After getting the primer,Use the K-up and K-do to detect the P+K+C. Meanwhile use the digestion protocol to ensure the result.