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Quorum addiction module

• The minipreps done on the 26 contained some genomic DNA, so we purified them on gel. Sadly, the purification didn't work, all the DNA was lost.
• Made the minipreps again.
• First ligations of the various pieces to build the two plasmids.

Module hydrogen

• The test on the other deletion candidate are once again all negative
• We continue the purification process for the deletion candidate
• We continue to try to obtain other deletion candidates

Module homologous recombination

• We inserted the cm and kan resistance cassette into the pSB1C3

Module heavy metals detection

• We inserted the gene of the carotene behind a constitutive promoter, we let the bacteria grow
• We inserted the copper sensitive promoter in front of a RFP, we let the bacteria grow

Other

• We did a PCR on pSB1C3 in order to have some more