Team:Groningen/11 October 2010
From 2010.igem.org
Laura
Debugging the matlab model for gene expression and killswitch. Find more information about constants used in the gene expression model.
Peter & David
Expression experiment on new transformants: E+H1, E+H2, C1, C2, E and H mix 4 clones.
Growing the strains from overnight cultures in 5 ml of TY medium at 37 degrees.
David
Biofilm paste experiment
Growing biofilms on sterilized ceramics that have been coated with athin layer of TY medium with corn starch.
Mix: 30 mg cornstarch/ml TY 1% agar medium and 0.5% glucose
After dissolving the agar and addition of corn starch teh pieces of cermics we added an taken out
Expression experiment:
ThT measurement of EH induced together with C non induced(this due to a mixup during he experiment), EH non induced, C induced