Team:TU Delft/12 July 2010 content

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Lab Work

Characterization of Anderson RBS sequences

E.coli TOP 10 strains containing the following composite biobricks in pSB1A2 had been obtained:

BioBrick #	Composed of
[http://partsregistry.org/Part:BBa_K398050]	J23100 + J61100 + I13401
K398051	J23100 + J61101 + I13401
K398052	J23100 + J61107 + I13401
K398053	J23100 + J61117 + I13401
K398054	J23100 + J61127 + I13401

To prepare for the fluorescence assay measurements the strains were grown in M9 minimal medium containing 20% glucose and ampicillin.

High copy number RBS characterizations are rarely indicative of RiPS in system operation plasmids, thus the fluorescence assays will also be performed with the biobricks on pSB3C5. In order to obtain sufficient BioBrick DNA the strains were grown in 250 mL LB-Amp, awaiting isolation and plasmid swapping.