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From 2010.igem.org

Chair: Da, Minutes: Younus, Comp

1. . Cloning Strategies

• SR1 - Finished
• RocF not started - need to use Gibson method;
• YneA
• 2 for Swarming (sfp and swrA)
• LacI
• 2 for Subtilin (Immunity and Production)

Only finished one, SR1. 7 more to go!

Next: swarming and subtilin nearly finished, YneA nearly coming back and LacI brick not sure how to test. Why is testing LacI hard?

• YneA - our most important BB - Phil, Rach, Deena, Younus
• Swarming (2) - Harsh, Alan, Steve, Da
• LacI - Phil, Younus, Rachel
• Subtilin (2) - Harsh, Alan, Steve, Deena
• RocF - Harsh, Alan, Steve, Da

5 parts to a Cloning Strategy

1. Purpose and justification
2. Modelling
3. Construction
4. Cloning and Integration
5. Testing and Characterising

1. . Wiki

Labbook

1. . Aim
2. . Materials and Methods
3. . Procedure/Protocol
4. . Results
5. . Conclusion

Everyone needs to carry out this protocol above Photographs add and label them In charge of wiki - Deena and Rachel (everyone everyday they have to send the links to both of them to check)

20th July - UK iGEM Gathering at Newcastle!

1. Presentation

Introduction - Rach, Deena, Jannetta

• Cover Page
• Picture of standing us up
• Introduction of our iGEM project - using BS to repair concrete (2 mins)

General Outlook (5 mins):

• Starting with bioreactor
• Transformation into spores
• Transportation
• Spraying on building
• Germination
• sensing crack + non-environmental kill switch
• swarming
• end of crack signalling = QS
• Filamentous cells
• CaCO3
• Glue
• Kill Switch

BioBrick Discussion (8 mins altogether; 2 mins each):

• Swarming
• Subtillin
• Filamentous cells - YneA
• CaCo3 - Urease

(5 mins remaining)

Whats next... 'Save the babies!!!'