Team:Newcastle/21 June 2010

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Monday

Contents 1 Wet Lab techniques practice 1.1 Aims 1.2 Equipement list 1.3 Tips 1.4 Streak plating 1.5 Set up culture for minipreps

Wet Lab techniques practice

Aims

The aim of today's Lab training session was to practice aseptic technique and wet lab techniques such as streak plating and broth culture.

Equipement list

For today's session we needed:

• Agar plates
• Pipettes
• Wire loops
• E.coli (from a colony)
• LB broth
• Bunsen burner
• Conical flasks
• Orbital shaker

Tips

• Use Aseptic technique: Treat everything as a pathogen!
• Work around the bunsen burner.
• Use a control.
• Clean the bench at the end of the day!
• Heat the wire loop from the middle to the tip.
• Keep plates upside down.
• Keep lids off for as short a time as possible.
• Loosen all the tops off the vessels before you start.
• Flame tops of bottles lightly.

Streak plating

Star plate streaking student, Deena, shows off how it is to be done!

Set up culture for minipreps

5ml culture with E.coli from a single colony grown over night. Tomorrow we will harvest the DNA.