Team:Harvard/parts
From 2010.igem.org
parts
notebook notation | biobrick name | original name | description | resistance (bacteria) | resistance (plants) | sequence |
V7 | BBa_K382000 | pORE Open Series Vector with BioBrick Sites | Agrobacterium vector open series with biobrick cloning site | kan | pat | link |
V8 | BBa_K382001 | pORE Open Series Vector with BioBrick Sites | Agrobacterium vector open series with biobrick cloning site | kan | nptII | link |
V9 | BBa_K382002 | pORE Expression Series Vector with BioBrick Sites | Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site | kan | pat | link |
V10 | BBa_K382003 | pORE Expression Series Vector with BioBrick Sites | Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site | kan | nptII | link |
V11 | BBa_K382004 | pORE Reporter Series Vector with BioBrick Sites | Agrobacterium vector with gusA reporter and biobrick cloning site | kan | nptII | link |
V12 | BBa_K382005 | pORE Reporter Series Vector with BioBrick Sites | Agrobacterium vector with smgfp reporter and biobrick cloning site | kan | nptII | link |
C1 | BBa_K382010 | amiRNA LUT2 | amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown | chloramphenicol | - | link |
C2 | BBa_K382011 | amiRNA BETA-OHASE 1 | amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown | chloramphenicol | - | link |
C3 | BBa_K382012 | amiRNA LYC | amiRNA construct for lycopene beta cyclase (LYC) knockdown | chloramphenicol | - | link |
A2 | BBa_K382014 | ihpRNA Bet | Intron hairpin Bet Knockdown construct | amp | - | link |
A3 | BBa_K382015 | ihpRNA LTP | Intron hairpin LTP Knockdown construct | chloramphenicol | - | link |
BBa_K382017 | amiRNA LTP | LTP knockdown construct | amp | - | link | |
BBa_K382018 | PDK intron hairpin | Intron hairpin construct | amp | - | link | |
BBa_K382019 | ihpRNA Ger | Intron hairpin Ger Knockdown construct | chloramphenicol | - | link | |
F1 | BBa_K382020 | Arabidopsis-optimized Brazzein | Sweet-tasting protein. 54 amino acid sequence. | chloramphenicol | - | link |
F2 | BBa_K382021 | Arabidopsis-optimized Miraculin | Taste-inverting protein turns sour to sweet. 191 amino acid sequence | chloramphenicol | - | link |
F3 | BBa_K382022 | pENTCUP2 promoter | Plant specific promoter. Used to drive expression of our flavor constructs in plants. | chloramphenicol | - | link |
F4 | BBa_K382023 | NOSt terminator | Plant specific terminator. Terminates transcription but no stop codon on end. | chloramphenicol | - | link |
F5 | BBa_K382024 | NOSt terminator + stop | Plant specific terminator. Terminates transcription with stop codon on 5' end. | chloramphenicol | - | link |
F6 | BBa_K382025 | Arabidopsis-optimized Miraculin StrepII N-terminus | Miraculin with StrepII tag on N-terminus for Western Blot. | chloramphenicol | - | link |
F7 | BBa_K382026 | Arabidopsis-optimized Brazzein StrepII N-terminus | Brazzein with StrepII tag on N-terminus for Western Blot. | chloramphenicol | - | link |
F8 | BBa_K382027 | Arabidopsis-optimized Miraculin StrepII C-terminus | Miraculin with StrepII tag on C-terminus for Western Blot. | chloramphenicol | - | link |
F9 | BBa_K382028 | Arabidopsis-optimized Brazzein StrepII C-terminus | Brazzein with StrepII tag on C-terminus for Western Blot. | chloramphenicol | - | link |
E2 | BBa_K382030 | RXRHm | Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide. | chloramphenicol | - | link |
E3 | BBa_K382031 | RXRLc | Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide | amp | - | link |
E5 | BBa_K382033 | Ecdysone Receptor | Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide | chloramphenicol | - | link |
E6 | BBa_K382034 | Act2pLacO | Actin promoter with LacO sites | amp | - | link |
BBa_K382035 | LacIN | Lac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoter | chloramphenicol | - | link | |
BBa_K382036 | RXRHm | Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide. | amp | - | link | |
BBa_K382037 | Ecdysone Receptor | Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide | amp | - | link | |
BBa_K382040 | Arabidopsis-optimized Miraculin YFP2x C-terminus | Miraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression | chloramphenicol | - | link | |
BBa_K382041 | Arabidopsis-optimized Brazzein YFP2x C-terminus | Brazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression | chloramphenicol | - | link | |
BBa_K382050 | Arabidopsis-optimized Miraculin | Taste-inverting protein turns sour to sweet. 191 amino acid sequence | kan | pat | link | |
BBa_K382051 | Arabidopsis-optimized Miraculin | Taste-inverting protein turns sour to sweet. 191 amino acid sequence | kan | nptII | link | |
BBa_K382052 | Arabidopsis-optimized Brazzein | Sweet-tasting protein. 54 amino acid sequence. | kan | pat | link | |
BBa_K382053 | Arabidopsis-optimized Brazzein | Sweet-tasting protein. 54 amino acid sequence. | kan | nptII | link | |
BBa_K382054 | amiRNA GFP | amiRNA construct for GFP knockdown | kan | pat | link | |
BBa_K382055 | amiRNA GFP | amiRNA construct for GFP knockdown | kan | nptII | link | |
BBa_K382058 | amiRNA LUT2 | amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown | kan | pat | link | |
BBa_K382059 | amiRNA BETA-OHASE 1 | amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown | kan | pat | link | |
BBa_K382060 | amiRNA LYC | amiRNA construct for lycopene beta cyclase (LYC) knockdown | kan | pat | link | |
BBa_K382061 | amiRNA LUT2 | amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown | kan | nptII | link | |
BBa_K382062 | amiRNA BETA-OHASE 1 | amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown | kan | nptII | link | |
BBa_K382063 | amiRNA LYC | amiRNA construct for lycopene beta cyclase (LYC) knockdown | kan | nptII | link | |
BBa_K382064 | amiRNA LTP | LTP knockdown construct | kan | pat | link | |
BBa_K382065 | amiRNA LTP | LTP knockdown construct | kan | nptII | link | |
BBa_K382066 | ihpRNA Ger | Intron hairpin Ger Knockdown construct | kan | pat | link | |
BBa_K382067 | ihpRNA Ger | Intron hairpin Ger Knockdown construct | kan | nptII | link | |
BBa_K382068 | ihpRNA BetV1 | Intron hairpin Bet Knockdown construct | kan | pat | link | |
BBa_K382069 | ihpRNA BetV1 | Intron hairpin Bet Knockdown construct | kan | nptII | link | |
BBa_K382070 | ihpRNA LTP | Intron hairpin LTP Knockdown construct | kan | pat | link | |
BBa_K382071 | ihpRNA LTP | Intron hairpin LTP Knockdown construct | kan | nptII | link |