Checked concentration of DNA used yesterday for ligation

• 4 ng/uL RFP digested using either H buffer and M buffer
• pSB1C3 band was very week

Ethanol precipitation

In accordance to Mighty Mix default protocol that for ligation DNA must melted in TE and for concentrating it more Ethanol precipitation was done.

Colony PCR of competent cells

Previous colony PCR produced strange bands so checked if there were from DNA of competent cell

• Colony PCR didn't produce any bands so those bands had to be an artifact from plasmid used for transformation