From 2010.igem.org

Week #6

11th October - 15 October 2010

Monday

We kept inoculated cells on LB liquid medium at 37ºC overnight, each containing 30ml.

Prepared miniprep for I20260 and E0240, we are going to work on the characterize the promoter

and the cold expresion casstte by RPU's.

Tuesday

Yesterday's overnight inoculum we did miniprep and double digestion with EcoRI and PstI

To confirm the ligation of PCR 4